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Highly sensitive, precise, and versatile, Enzyme Linked Immunosorbent Assay (ELISA) is now the predominant assay used in biomedical and agricultural research, as well as in applied science. In The ELISA Guidebook, John R. Crowther provides todayís premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.

57.083.34 --- Fluorescent antibody tests. ELISA --- 57.083.34 Fluorescent antibody tests. ELISA --- Enzyme-linked immunosorbant assay --- Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- Enzyme-linked immunosorbent assay

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DNA repair has assumed a new importance with the discovery that malfunctioning of the DNA repair pathways in humans can lead to many disease states. In DNA Repair Protocols: Prokaryotic Systems, well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Each readily reproducible protocol is presented by a hands-on expert in sufficient detail to ensure robust experimental results and is supplemented by chapter introductions, as well as notes offering a wealth of interesting and useful information. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology.

DNA repair --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Biochemical genetics. --- Chemical genetics --- Biochemistry --- Molecular genetics --- Deoxyribonucleic acid repair --- Repair, DNA --- Repair mechanisms in DNA --- Biochemical genetics --- Antimutagens --- Cytology.

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Stap voor stap hypothese voor het ontstaan van een cel. Duidelijke opbouw.

Evolution (Biology) --- Cells --- Biological Processes --- Genetic Processes --- Biological Phenomena --- Genetic Phenomena --- Phenomena and Processes --- Biological Evolution --- Biology --- Health & Biological Sciences --- Evolution --- 573.5 --- 575.8 --- Golgi-apparaat --- biologie --- celkernen --- celleer --- chloroplasten --- cytologie --- eukaryoten --- evolutie --- evolutiemechanismen --- flagellen --- membranen --- mitochondriën --- prokaryoten --- Animal evolution --- Animals --- Biological evolution --- Darwinism --- Evolutionary biology --- Evolutionary science --- Origin of species --- Biological fitness --- Homoplasy --- Natural selection --- Phylogeny --- Cell evolution --- Cellular evolution --- Life sciences. --- Medicine. --- Life Sciences, general. --- Biomedicine, general. --- Health Workforce --- Biosciences --- Sciences, Life --- Science --- EVOLUTION --- LIFE --- PROKARYOTES --- EUKARYOTES --- FLAGELLA --- PLASMA MEMBRANE --- NUCLEUS --- DIFFERENTIATION --- GOLGI APPARATUS --- MITOCHONDRIA --- PLASTIDS --- CILIA --- ORIGIN

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In this Journey to Microbial Worlds we present the diversity of microorganisms, from the state of fossil microbes in Archaean age rocks to the possibilities of extraterrestrial life. This volume discusses the extremophiles living in harsh environments (from our anthropocentric point) and describes them in considerable detail. Some chapters also review topics such as symbiosis, bacterial luminescence, methanogens, and petroleum-grown cells. The final chapters of this book shed new light on astrobiology and speculate on extremophiles as candidates for extraterrestrial life. All chapters are updated to the latest research level.

Biology --- Health & Biological Sciences --- Biology - General --- Extreme environments --- Adaptation (Physiology) --- Public Health --- Microbiology --- Physiological Processes --- Adaptation, Biological --- Environment and Public Health --- Physiological Phenomena --- Biological Processes --- Biological Science Disciplines --- Biological Phenomena --- Health Care --- Phenomena and Processes --- Natural Science Disciplines --- Disciplines and Occupations --- Adaptation, Physiological --- Environmental Microbiology --- Microbiology & Immunology --- Microbiology. --- Life sciences. --- Plant science. --- Botany. --- Aquatic ecology . --- Oceanography. --- Life Sciences, general. --- Plant Sciences. --- Freshwater & Marine Ecology. --- Oceanography, Physical --- Oceanology --- Physical oceanography --- Thalassography --- Earth sciences --- Marine sciences --- Ocean --- Aquatic biology --- Ecology --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Natural history --- Plants --- Biosciences --- Sciences, Life --- Science --- Microbial biology --- Microorganisms --- Floristic botany

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Nature Reviews Genetics is an invaluable source of information in genetics and genomics. The journal’s scope covers the whole breadth of these and related fields, bringing readers cutting-edge Reviews on topics that range from molecular genetics to evolution to systems biology. By publishing Reviews, Progress, Comment, Analysis and Perspective articles, among which are Viewpoints from opinion-leaders, Nature Reviews Genetics provides a balanced and unique perspective of this exciting field which goes well beyond the conventional review, and appeals to students and established scientists alike. All articles are written by carefully chosen leaders in their field and subject to rigorous peer-review, resulting in each issue providing balanced, high-quality and authoritative articles. While experts appreciate the insights and thought-provoking syntheses provided by the high-calibre authors, non-specialists are helped by the glossary definitions, additional background information in the boxes and highlighted references.

Genetics --- Génétique --- Periodicals. --- Périodiques --- Genetics. --- Genetic Processes --- Genetic Structures --- Genetic Phenomena --- Periodicals --- Health Sciences --- Information Technology --- Life Sciences --- Medical Education, Training & Research --- Computational Biosciences --- Biology --- General and Others --- Génétique --- Périodiques --- EBSCOASP-E EJMEDEC EPUB-ALPHA-N EPUB-PER-FT MDGENETI NPG-E --- MDGENETI --- medische genetica --- genetica --- genexpressie --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Medicine --- Cancer research --- Human genetics --- Medical genetics --- Agriculture --- Biomedicine, general --- Human Genetics --- Cancer Research --- Gene Function --- Animal Genetics and Genomics --- Farming --- Husbandry --- Industrial arts --- Life sciences --- Food supply --- Land use, Rural --- Clinical genetics --- Diseases --- Heredity of disease --- Medical sciences --- Pathology --- Genetic disorders --- Heredity, Human --- Human biology --- Physical anthropology --- Health Workforce --- Genetic aspects